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product name
Hepa-1c1c7, 95090613
biological source
mouse liver
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Aryl hydrocarbon
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse hepatoma
Cell Line Description
Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established.
Application
Study of Ah receptors, P450IA1 regulation
Culture Medium
α MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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