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Key Documents

DH82

94062922, dog blood, Macrophage

Synonym(s):

DH82 Cell Line

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About This Item

UNSPSC Code:
41106514

product name

DH82, 94062922

biological source

dog blood

growth mode

Suspension

karyotype

Not specified

morphology

Macrophage

products

Not specified

receptors

FC-gamma receptors

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Canine Monocyte-macrophage

Cell Line Description

Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Maintain cultures at 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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