DKB human cell line

88052074, IHW Number: 9075

• UNSPSC Code: 41106514

Properties

• biological source: human blood
• ethnicity: Dutch
• gender: female
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

General description

Consanguineous: Yes

Homozygous: Yes

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 2402
Cw: 304
B: 4001
HLA DRA: 101
HLA DRB1: 9012
HLA DRB4: 103
HLA DQAP: 3.2
HLA DQA1: 302
HLA DQB1: 3032
HLA DPA1: 1
HLA DPB1: 401

Serological Profile

HLA A: 24
HLA A ief: 24.1
HLA C: 10
HLA B: 60
HLA B ief: 60
Bw4/bw6: 6
HLA Dw: 23
HLA DR: 9
HLA DR53: 53
HLA DQ: 9
HLA DP: 4

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.