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Key Documents

Hs 578T

86082104, human breast, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

Hs 578T, 86082104

biological source

human breast

growth mode

Adherent

karyotype

2n = 46

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human breast carcinoma

Cell Line Description

Tumorigenic in immunosuppressed mice and form colonies in semisolid medium. Neither oestrogen receptors nor endogenous viruses were detected.

Application

Hs 578T has been used to measure the calcium-dependent binding of cadherin-11 and N-cadherin. It has also been used to study the changes in gene expression induced by insulin-like growth factor-binding protein (IGFBP-2).

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17

Culture Medium

DMEM + 2mM Glutamine + 10μg/ml Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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