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product name
RFL-6, 86072501
biological source
rat lung
growth mode
Adherent
karyotype
2n = 42
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Sprague-Dawley lung
Cell Line Description
Derived from the lung tissue of a normal germ-free, 18 day gestation, Sprague-Dawley rat foetus. At least 45 population doublings expected.
Culture Medium
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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