h1RPE7

9061602, human eye, Polygonal

• UNSPSC Code: 41106514

Properties

• product name: h1RPE7, 09061602, from human eye
• biological source: human eye
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

Cell Line Origin

An immortalized retinal pigment epithelial cell line

Cell Line Description

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Culture Medium

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.