KARPAS 299

6072604, human lymph, Lymphoblastoid

• Synonym(s): K299 Cells, KARPAS299 Cells
• UNSPSC Code: 41106514

Properties

• product name: KARPAS 299, 06072604
• biological source: human lymph
• growth mode: Suspension
• karyotype: Hypodiploid
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Non-Hodgkin′s Ki-positive Large Cell Lymphoma

Cell Line Description

A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin′s lymphoma in 1986.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Subculture Routine

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.