C4982

Cytochrome P450 human

3A4 isozyme microsomes, with P450 reductase and cytochrome b₅, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

• Synonym(s): Cytochrome P450 enzyme, Human cytochrome P450, P450 enzyme
• Enzyme Commission number: 1.6.2.4 (BRENDA, IUBMB)
• MDL number: MFCD03456520
• UNSPSC Code: 12161501
• NACRES: NA.47

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• form: solution
• mol wt: 45-60 kDa
• packaging: vial of ~0.5 nmol; vial of ≥25 units
• concentration: ≥2 mg/mL (Bichinchonic acid method)
• technique(s): co-immunoprecipitation (co-IP): suitable
• solubility: water: soluble
• suitability: suitable for molecular biology
• UniProt accession no.: P08684
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CYP3A4(1576)

Description

General description

Research area: IMMUNO AND CKS

Cytochrome P450 (CYP450) is a membrane-bound hemoprotein that constitutes a vast superfamily of heme-thiolate proteins engaged in the metabolism of exogenous and endogenous compounds. These CYP450 enzymes feature an active heme iron center that is bound to a protein molecule via a highly conserved cysteine thiolate ligand.

Application

Cytochrome P450 human has been used in in vitro binding assays to understand protein dimerization via haem–haem stacking and its significance in cancer. It has also been used to study in vitro binding between PGRMC1 and CYP3A4.

Biochem/physiol Actions

Cytochrome P450 (CYP) enzymes are associated with many reactions including O-dealkylation, epoxidation, S-oxidation, and hydroxylation.

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

Other Notes

Microsomes containing recombinant human CYP3A4 and recombinant rabbit NADPH-P450 reductase

Unit Definition

One unit will convert 1 nanomole of testosterone to 6β-hydroxytestosterone per minute at pH 7.4 at 37 °C.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable