50492
Atto Rho101 NHS ester
BioReagent, suitable for fluorescence
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About This Item
UNSPSC Code:
12352108
NACRES:
NA.32
Recommended Products
product line
BioReagent
Assay
90% (Coupling efficiency with benzylamine, HPLC)
form
solid
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm
586 nm
fluorescence
λex 586 nm; λem 610 nm
λ
in ethanol (0.1% trifluoroacetic acid)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Atto Rho101 NHS ester is a derivative of the well-known dye Rhodamine 101 for coupling to bio-molecules such as DNA, RNA or proteins. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO Rho101 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
Storage Class Code
11 - Combustible Solids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Journal of the American Chemical Society, 133(34), 13558-13564 (2011-08-09)
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Guang pu xue yu guang pu fen xi = Guang pu, 31(5), 1348-1351 (2011-08-02)
The absorption, fluorescence and time-resolved fluorescence spectra of Rhodamine 101 dye in both methanol and acidic methanol solutions were measured. The authors achieved the characteristic information of the absorption and fluorescence spectra, and obtained the S1 lifetimes. The authors assigned
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The determination of the fluorescence quantum yields (QY, Φ(f)) of a series of fluorescent dyes that span the absorption/excitation and emission ranges of 520-900 and 600-1000 nm is reported. The dyes encompass commercially available rhodamine 101 (Rh-101, Φ(f) = 0.913)
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