49767

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

• Synonym(s): 1,2,3-Propanetriol, Glycerin
• Linear Formula: HOCH₂CH(OH)CH₂OH
• CAS Number: 56-81-5
• Molecular Weight: 92.09
• Beilstein: 635685
• EC Number: 200-289-5
• MDL number: MFCD00004722
• UNSPSC Code: 50161901
• PubChem Substance ID: 57651220
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 3.1 (vs air)
• vapor pressure: <1 mmHg ( 20 °C)
• product line: BioUltra
• Assay: ≥99.5% (GC)
• form: viscous liquid
• autoignition temp.: 698 °F
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.1%
• refractive index: n20/D 1.474 (lit.); n20/D 1.474
• pH: 5.5-8 (25 °C, 5 M in H₂O)
• bp: 182 °C/20 mmHg (lit.)
• mp: 20 °C (lit.)
• solubility: H₂O: 5 M at 20 °C, clear, colorless
• density: 1.25 g/mL (lit.)
• anion traces: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤5 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• SMILES string: OCC(O)CO
• λ: 5 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.07
• InChI: 1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
• InChI key: PEDCQBHIVMGVHV-UHFFFAOYSA-N

Description

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol has been used as

• a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• a fuel during designing enzymatic biofuel cell.
• a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
• a matrix for fast atom bombardment MS.
• may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Other Notes

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 390.2 °F - Pensky-Martens closed cup
• Flash Point(C): 199 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves