46985

Fluorescein-5-thiosemicarbazide

suitable for fluorescence, ~80% (HPCE)

• Empirical Formula (Hill Notation): C₂₁H₁₅N₃O₅S
• CAS Number: 76863-28-0
• Molecular Weight: 421.43
• MDL number: MFCD00155658
• UNSPSC Code: 12352125
• PubChem Substance ID: 329757284
• NACRES: NA.32

Properties

• Assay: ~80% (HPCE)
• form: solid
• solubility: DMF: soluble; H₂O: soluble
• fluorescence: λ_ex 492 nm; λ_em 516 nm in 0.1 M Tris pH 9.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: NNC(=S)Nc1ccc(c(c1)C(O)=O)C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24
• InChI: 1S/C21H15N3O5S/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19/h1-9,25H,22H2,(H,27,28)(H2,23,24,30)
• InChI key: MEUCHQDLZYLNQY-UHFFFAOYSA-N

Description

General description

Fluorescein-5-thiosemicarbazide, also known as FTC, is a fluorescent probe. The FTSC fluorescent spectra is like FITC, a preferred fluorescent tag for proteomics. The fluorescence spectral properties of FTSC-labeled vesicles obtained by reductive amination are fully compatible with the argon laser of fluorescent instruments.

Application

Fluorescein-5-thiosemicarbazide can be used as a fluorescent labeling reagent to label:

• Cell-surface functional groups (glycophorins) in the study of the effect of deoxygenation in the red blood cell membrane.
• Saccharides applicable in polysaccharide imaging in live cells.
• Chondroitin sulfate nanogels applicable in cell-specific drug delivery applications.

Features and Benefits

Fluorescein-5-thiosemicarbazide has the following benefits -

• It displays strong fluorescence property.
• Shows an intrinsic reactivity of the thiosemicarbazide group towards the aldehyde group.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves