T17917

Tricine

≥99% (titration), reagent grade, crystalline

• Synonym(s): N-[Tris(hydroxymethyl)methyl]glycine
• Linear Formula: (HOCH₂)₃CNHCH₂CO₂H
• CAS Number: 5704-04-1
• Molecular Weight: 179.17
• Beilstein: 1937804
• MDL number: MFCD00004277
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• biological source: synthetic
• Quality Level: 200
• grade: reagent grade
• Assay: ≥99% (titration)
• form: crystalline
• storage condition: (Tightly closed. Dry)
• impurities: <0.5% water
• color: white
• useful pH range: 7.4-8.8
• pKa (25 °C): 8.1
• mp: 187 °C
• solubility: water: 0.1 M, clear, colorless
• cation traces: Pb: ≤5 ppm
• storage temp.: room temp
• SMILES string: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChI key: SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

General description

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Application

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

Buffer component for separation of low molecular weight peptides.

Features and Benefits

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable