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Key Documents

PPB020

Sigma-Aldrich

SSC Buffer

pH 7.0, pHast Pack, powder

Synonym(s):

1X SSC buffer, 1X SSC hybridization buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

product line

pHast Pack

Quality Level

form

powder

pH

7.0

Application

Saline sodium citrate buffer is commonly used as a wash buffer for Nucleic acid hybridization techniques like Northern and Sothern blotting.

Features and Benefits

  • Ready-to-use buffer
  • No measuring and pH adjusting needed
  • Save time and minimize effort
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl and 15 mM Na3C6H5O7, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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