PPB011

Tris Buffered Saline

powder, pH 7.6, pHast Pack^™

• Synonym(s): TBS
• MDL number: MFCD00132476
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• product name: Tris Buffered Saline, pH 7.6, pHast Pack^™, powder
• product line: pHast Pack^™
• Quality Level: 100
• form: powder
• storage condition: (Tightly closed. Dry)
• impurities: ≤5 ppm Heavy Metals (lead)
• color: white
• pH: 7.6
• solubility: soluble, clear, colorless
• cation traces: Fe: ~10 ppm
• foreign activity: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• storage temp.: room temp

Description

General description

Tris Buffered Saline, or TBS, pH 7.6 is a commonly used wash buffer that is isotonic and non-toxic and ideal for use in cell culture and electrophoresis applications.

Application

Tris-buffered saline can be used as a wash buffer for:

• Enzyme Linked Immunosorbent assay (ELISA)
• Western Blot and other protein applications
• Suitable for use in cell culture (recommend to sterile filter)
• Immunohistochemistry
• Sample preparation
• Resuspension and sonication of cells

Features and Benefits

• Ready for use, just dissolve in ultrapure water
• No calculations, measuring, and pH adjustments needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl, pH 7.6 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture applications.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1