F7258

Fast Green FCF

certified by the Biological Stain Commission

• Synonym(s): Food green 3
• Linear Formula: C₃₇H₃₄N₂O₁₀S₃Na₂
• CAS Number: 2353-45-9
• Molecular Weight: 808.85
• Colour Index Number: 42053
• Beilstein: 5718212
• EC Number: 219-091-5
• MDL number: MFCD00013053
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894936
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: dye content, ≥90%
• mp: 290 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
• InChI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• InChI key: RZSYLLSAWYUBPE-UHFFFAOYSA-L

Description

Application

Fast Green FCF has been used:

• along with safranin for the staining of nodule sections
• in the collagen deposit staining procedure
• for the staining of protein and starch
• as a dye to detect and inject DNA solution in chicken embryo

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Suitability

Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H335,H341
• Precautionary Statements: P202 - P261 - P271 - P280 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Muta. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): 352.4 °F - closed cup
• Flash Point(C): 178 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves