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DIGUTP-RO

Roche

Digoxigenin-11-UTP

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.21
Pricing and availability is not currently available.

Assay

≥85% (HPLC)

Quality Level

100

form

solution

mol wt

(Mr = 1106.7 (DIG-11-UTP-Li4))

packaging

pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases.[1][2] It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:
  • in situ hybridization[3][4][5]
  • in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)[6]
  • fluorescence in situ hybridization (FISH)[7]

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Quality

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C43H61N4O22P3Li4

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
60007521
33812621
33812620
63347020
60007520

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A George et al.
Connective tissue research, 33(1-3), 67-72 (1995-01-01)
Acidic phosphorylated proteins are prominent constituents of the extracellular matrix of bone and dentin. It has been postulated that they may have important structural and regulatory roles in the process of tissue mineralization. Studies of a cDNA library, prepared from
Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)
Isolation and functional characterization of two dioxygenases putatively involved in bixin biosynthesis in annatto (Bixa orellana L.)
<BIG>Carballo U, et al.</BIG>
PeerJ, 7 (2019)
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution
<BIG>Chen X, et al.</BIG>
Biotechniques, 56 (2014)
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