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11796895001

Roche

DIG Easy Hyb Granules

greener alternative

powder, suitable for hybridization

Synonym(s):

DIG system

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About This Item

UNSPSC Code:
41105326

Agency

suitable for

Quality Level

form

powder

usage

sufficient for 6 x 100 mL

manufacturer/tradename

Roche

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

hybridization: suitable

color

white

pH

7,0

solubility

water: soluble

suitability

suitable for nucleic acid hybridization

greener alternative category

storage temp.

20-25°C

General description

DIG Easy Hyb is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.

Features and Benefits

  • DIG Easy Hyb is a convenient ready-to-use hybridization buffer, saving time and effort.
  • DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.

Preparation Note

Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

DIG Easy Hyb is a trademark of Roche

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ru-Bing Duan et al.
The Journal of biological chemistry, 289(23), 16006-16015 (2014-04-24)
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Retrotransposons are mobile genetic elements capable of transposition via reverse transcription of RNA intermediates. Rice cultivar Nipponbare contains two nearly identical genomic copies of Tos17, an endogenous copia-like LTR retrotransposon, on chromosomes 7 (Tos17 Chr.7) and 10 (Tos17 Chr.10), respectively.
Chih-Chiang Chan et al.
Current biology : CB, 21(20), 1704-1715 (2011-10-18)
Neurons require highly specialized intracellular membrane trafficking, especially at synapses. Rab GTPases are considered master regulators of membrane trafficking in all cells, and only very few Rabs have known neuron-specific functions. Here, we present the first systematic characterization of neuronal
An Li et al.
Fungal genetics and biology : FG & B, 48(6), 602-611 (2011-02-18)
Several Aspergillus species are well-known for the production of a variety of organic acids. In this study, a cloned based transcriptomics approach was used to identify genes crucial in the biosynthesis pathway for one of these acids, itaconic acid. From
Ziye Rong et al.
Translational vision science & technology, 9(9), 47-47 (2020-09-17)
Fuchs' endothelial corneal dystrophy (FECD) is the leading indication for corneal transplantation. Seventy percent of cases are caused by an intronic CTG triplet repeat expansion in the TCF4 gene that results in accumulation of pathogenic expanded CUG repeat RNA (CUGexp)

Protocols

Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting

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