SCC169
EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line
Mouse
Synonym(s):
JK1 Feeder Cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
EmbryoMax® JK1 Murine Testicular Stromal Feeder Cell Line, Immortalized Mouse Feeder Cell Line
biological source
mouse
manufacturer/tradename
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
shipped in
ambient
General description
JK1 is an immortalized CD34+SMA+ cell line derived from spontaneous transformation of C57/BL6 mouse testicular stromal cells. JK1 cells are able to support the long-term culture of a variety of primitive cells including multi-potent adult spermatogonial-derived stem cells, pluripotent murine embyronic stem cells and embyronic germ cells derived from primordial germ cells. Adult spermatogonial stem and progenitor cells (SPCs) cultured on JK1 feeder cells express key features of germ line stem cells including expression of PLZF, DAZL, and GCNA1. JK1 feeder cells has maintained its capacity for promoting stem cell self-renewal even after serial passaging for over one year.
Cell Line Description
Fibroblast Cells
Application
Immortalized Mouse Feeder Cell Line
Research Category
Stem Cell Research
Stem Cell Research
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Quality
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.
Storage and Stability
EmbryoMax JK1 Murine Testicular Stromal Feeder Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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