MABT848

Anti-MYH-2 Antibody, clone 8F72C8

clone 8F72C8, from rat

• Synonym(s): Myosin-2, Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain IIa, MyHC-IIa, Myosin heavy chain, skeletal muscle, adult 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8F72C8, monoclonal
• species reactivity: rat
• species reactivity (predicted by homology): mouse, human (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001093582
• UniProt accession no.: Q9UKX2
• target post-translational modification: unmodified
• Gene Information: human ... MYH2(4620)

Description

General description

Myosin-2 (UniProt: Q9UKX2; also known as Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain IIa, MyHC-IIa, Myosin heavy chain, skeletal muscle, adult 2) is encoded by the MYH2 (also known as MYHSA2) gene (Gene ID: 4620) in human. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-2 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-784), and an IQ domain (aa 787-816). It also contains 2 actin binding regions (aa 661-683 and 763-777). Mutations in MYH2 gene are known to cause Proximal myopathy and ophthalmoplegia (MYPOP), a muscular disorder characterized by mild-to-moderate muscle weakness and predominance of type 1 fibers and small or absent type 2A fibers.

Specificity

Clone 8F72C8 is a rat monoclonal antibody that detects Myosin-2 in mouse and rat muscle. It targets an epitope within 29 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of human Myosin-2

Application

Anti-MYH-2, clone 8F72C8, Cat. No. MABT848, is a rat monoclonal antibody that detects Myosin-2 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected MYH-2 in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Western Blotting Analysis: A representative lot detected MYH-2 in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunofluorescence Analysis: A representative lot detected MYH-2 in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-2 in mouse heart and mouse skeletal muscle tissues.

Quality

Evaluated by Western Blotting in mouse soleus muscle tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-2 in mouse soleus muscle tissue lysate.

Target description

~220 kDa observed; 223.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1