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MABN1580

Sigma-Aldrich

Anti-beta-Arrestin-2 Antibody, clone 5E12.1

clone 5E12.1, from mouse

Synonym(s):

Arrestin beta-2, beta-Arrestin-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5E12.1, monoclonal

species reactivity

human, rat

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ARRB2(409)

General description

Beta-arrestin-2 (UniProt P32121; also known as Arrestin beta-2) is encoded by the ARRB2 (also known as ARB2, ARR2, BARR2) gene (Gene ID 409) in human. β-Arrestin 1 and 2 are cytosolic adapter proteins originally identified as negative regulators of G protein-coupled receptor (GPCR) signaling through receptor desensitization and internalization. It is now known that these multifunctional scaffold proteins also elicit G-protein-independent signalling pathways by linking the activated GPCRs to multiple signaling molecules, such as ERK1/2, Akt, and Src kinases. In addtion to GPCRs, β-arrestins are also involved in receptor tyrosine kinase (RTK) signalling pathways, and β-arrestin-2 is reported to link Src and Akt to insulin receptor (IR) to ensure a full Akt activation. Although β-arrestin-1 and β-arrestin-2 share a high degree of structural homology, they do display specific functions in several physiological processes. β-arrestins can adopt multiple conformations to display distinct sets of interacting domains. Recruitment of β-arrestins to a specific GPCR results in the formation of distinct β-arrestin scaffolds, allowing a high level of specificity for a given agonist.

Specificity

Expected to react with spliced isoforms 1, 4, and 5 reported by UniProt (P32121). Reactivity toward spliced isoforms 2 & 3 has not been determined.

Immunogen

Epitope: C-terminal region.
KLH-conjugated linear peptide corresponding to a sequence in the C-terminal region of human beta-Arrestin-2.

Application

Anti-beta-Arrestin-2 Antibody, clone 5E12.1 is an antibody against beta-Arrestin-2 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected beta-Arrestin-2 in rat testis and human cerebral cortex & liver tissues.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected beta-Arrestin-2 in 200 µg of HeLa cell lysate.

Target description

~52 kDa observed. 46.11 kDa (isoform 1), 47.27 kDa (isoform 2), 45.56 kDa (isoform 3), 48.02 kDa (isoform 4), and 4439 kDa (isoform 5) calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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