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MABE1985

Sigma-Aldrich

Anti-Gemin5 Antibody, clone 1E12

Synonym(s):

Gem-associated protein 5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1E12, monoclonal

mol wt

calculated mol wt 168.59 kDa
observed mol wt ~170 kDa

purified by

using protein G

species reactivity

human, human, monkey

species reactivity (predicted by homology)

porcine

packaging

antibody small pack of 100 μg

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

shipped in

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GEMIN5(25929)

General description

Gem-associated protein 5 (UniProt: Q8TEQ6; also known as Gemin5) is encoded by the GEMIN5 gene (Gene ID: 25929) in human. Survival of motor neurons (SMN) protein is known to form a stable complex with a group of proteins named gemins. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs) and thereby plays an important role in the splicing of cellular pre-mRNAs. Gemin5, a highly conserved, multi-functional protein, is a component of the core SMN complex and is found in the nucleoplasm and nuclear bodies known as Gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. It is also abundantly present in the cytoplasm. It plays a role in capturing snRNA into SMN complexes in the cytoplasm for transport into the nucleus. However, in the nucleus it is easily detached from the SMN-Gemin complex. Gemin5 is reported to physically bind to snRNA via a specific AU5-6 sequence located within the highly conserved Sm site and is flanked by a short stem loop that assists in delivery to the SMN complex. The specific snRNP code helps Gemin5 in distinguishing these snRNAs from other forms of cellular RNAs. Gemin5 contains 13 WD repeat domains that mediate its binding to U1 and U4 snRNA. WD domains are also reported to mediate binding to the 7-methylguanosine cap that is found on mRNA and snRNA molecules. Mutations in GEMIN5 gene are known to cause a distinct neurological cerebellar ataxia syndrome, through altered snRNP complex assembly. (Ref.: Kour, S., et al. (2021). Nat. Commun. 12; Article 2558; Hao, L., et al. (2007). BMC Cell Biol. 8:28).

Specificity

Clone 1E12 is a mouse monoclonal antibody that detects Gem-associated protein 5 (Gemin5). It targets an epitope within the C-terminal region.

Immunogen

His-tagged recombinant fragment corresponding to 292 amino acids form the C-terminal region of human Gem-associated protein 5 (Gemin5).

Application

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in COS-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in HeLa cell lysate.

Immunocytochemistry Analysis: A representative lot detected Gemin5 in Immunocytochemistry application (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28; Schilling, M., et al. (2021). Cell Rep. 35(12); 109277).

Western Blotting Analysis: A representative lot detected Gemin5 in Western Blotting applications (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Gemin5, clone 1E12 , Cat. No. MABE1985, is a mouse monoclonal antibody that detects Gemin5 and is tested for use in Immunocytochemistry and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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