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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6, monoclonal
mol wt
calculated mol wt 128.26 kDa
observed mol wt ~140 kDa
species reactivity
human, rat
packaging
antibody small pack of 100 μL
technique(s)
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... RFC1(5981)
General description
Replication factor C subunit 1 (UniProt: P35251; also known as Activator 1 140 kDa subunit, A1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1, DNA-binding protein PO-GA, Replication factor C 140 kDa subunit, RF-C 140 kDa subunit, RFC140, Replication factor C large subunit) is encoded by the RFC1 (also known as RFC140) gene (Gene ID: 5981) in human. Replication factor C (RFC) is a multi-subunit DNA replication factor that is composed of five subunits of 140, 40, 38, 37, and 36 kDa size. These five subunits exist in all eukaryotes and are highly conserved in both structure and function. RFC1 is the largest subunit in this complex. RFC serves as a DNA-dependent ATPase that binds in a structure-specific manner to the 3 end of a primer hybridized to a template DNA. This ATPase is activated in the presence of primer/template DNA and is further stimulated by PCNA addition. In eukaryotes, it acts as a clamp loader that can bind and open the homotrimeric PCNA clamp, and then load PCNA onto a template-primer junction in an ATP-dependent reaction. Coupled with ATP hydrolysis, RFC is ejected from the PCNA clamp for the next round of DNA synthesis and then the clamp recruits DNA polymerases for processive elongation of DNA chains. In addition to its role in DNA replication, RFC also plays a role in DNA polymerase switching, DNA repair, and check-point control in cell cycle progression. RFC1 displays wide tissue distribution but is undetectable in placental tissue. (Ref.: Chen, Y., et al. (2018). Front. Plant Sci. 9; 779; Bunz, F., et a. (1993). Proc. Natl. Acad. Sci. USA. 90 (23); 11014-11018; Tsurimoto, T., and Stillman, B. (1989). EMBO J. 8(12); 3883-3889).
Specificity
Clone 6 is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1).
Immunogen
Purified Replication factor C subunit 1 (RFC1) from HEK293 cell nuclear extract.
Application
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysates.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Replication factor C subunit 1 (RFC1) in HepG2 cell lysates.
Tested Applications
Western Blotting Analysis: A representative lot detected RFC1 in Western Blotting applications (Farina, A., et. al. (2008). J Biol Chem. 283(30):20925-36).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysates.
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Replication factor C subunit 1 (RFC1) in HepG2 cell lysates.
Tested Applications
Western Blotting Analysis: A representative lot detected RFC1 in Western Blotting applications (Farina, A., et. al. (2008). J Biol Chem. 283(30):20925-36).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-RFC1, clone 6, Cat. No. MABE1893, is a mouse monoclonal antibody that detects Replication factor C subunit 1 (RFC1) and is tested for use in Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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