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MABC1036

Sigma-Aldrich

Anti-EphA7 Antibody, clone 2F3.1

clone 2F3.1, from mouse

Synonym(s):

Ephrin type-A receptor 7, EHK-3, EK11, EPH homology kinase 3, EPH-like kinase 11, hEK11, Receptor protein-tyrosine kinase HEK11, Tyrosine-protein kinase receptor EHK-3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2F3.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EPHA7(2045)

General description

Ephrin type-A receptor 7 (EC 2.7.10.1; UniProt Q15375; also known as EHK-3, EK11, EPH homology kinase 3, EPH-like kinase 11, hEK11, Receptor protein-tyrosine kinase HEK11, Tyrosine-protein kinase receptor EHK-3) is encoded by the EPHA7 (also known as EHK3, HEK11) gene (Gene ID 2045) in human. EphA7 belongs to the ephrin receptor family of receptor tyrosine kinases that are activated upon ephrin binding. Ephrin receptor signaling plays important roles in embryonic development, tissue boundaries formation, angiogenesis, stem cell differentiation, and cancer. There exist nine type-A (EphA1-8 and EphA10) and five type-B (EphB1-4 and EphB6) ephrin receptors in human. EphA7 is initially produced with a signal peptide (a.a. 1-27) sequence, the removal of which yields the mature protein with a large extracellular (a.a. 28-555) region, followed by a transmembrane segment (a.a. 556-576) and a cytoplasmic region (a.a. 577-998) that contains the kinase domain (a.a. 633-894).

Specificity

Expected to react with all five spliced isoforms of human EphA7 reported by UniProt (Q15375).

Immunogen

Epitope: Extracellular domain.
GST-tagged recombinant extracellular N-terminal fragment of human EphA7.

Application

Anti-EphA7 Antibody, clone 2F3.1 is an antibody against EphA7 for use in Western Blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Cytokines & Cytokine Receptors

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected EphA7 in 10 µg of MCF-7 cell lysate.

Target description

~120 kDa observed. 112.1 kDa (isoform 1; EPHA7-FL), 111.5 kDa (isoform 2), 31.83 kDa (isoform 3), 111.6 kDa (isoform 4), and 50.52 kDa (isoform 5; EPHA7-S) calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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