MAB2079-AF647

Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor^™ 647 Conjugate

clone HUTS-4, from mouse, ALEXA FLUOR^™ 647

• Synonym(s): Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, Integrin β1, activated
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 647
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: HUTS-4, monoclonal
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable
• isotype: IgG2a
• NCBI accession no.: NM_002292.3
• UniProt accession no.: P05556
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGB1(3688)

Description

General description

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interacts with various integrin alpha subunits (including alpha 1, 2, 3, 4, 5 and 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Specificity

Monoclonal antibody HUTS-4 is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as HUTS-4 which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Antibody HUTS-4 recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.

Immunogen

Epitope: A.a. 355-425 region of the common β1 subunit of VLA integrins.

Generated against human beta1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in HeLa and A431 cells.
The unconjugated antibody (Cat. No. MAB2079Z) is shown to be suitable also for Western blotting, ELISA, functional assay, and immunohistochemistry.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Integrin β1, activated, clone HUTS-4, AlexaFluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of Integrin β1.

Quality

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin β1 in NIH/3T3 cells.

Target description

~88 kDa observed

Physical form

Protein G Purified

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable