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Sigma-Aldrich

Goat Anti-Human IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate

0.5 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

biotin conjugate

antibody form

purified antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

ELISA: suitable
immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Specificity

Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD.
The antibody reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.

Immunogen

Epitope: Human IgM, IgG, IgA
Prepared from pepsin digest of goat anti-human Ig.

Application

Goat anti-Human IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate is an antibody against Human IgM+IgG+IgA for use in ELISA & IF.
Immunofluorescence: ≤1 μg/106 cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies

Physical form

Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN3.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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