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Key Documents

ABD130

Sigma-Aldrich

Anti-PRDM16 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

PR domain containing 16, MEL1, PFM13, KIAA1675, MEL1, MDS1/EVI1-like gene 1, Transcription factor MEL1, PR domain zinc finger protein 16

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRDM16(63976)

General description

PR domain zinc finger protein 16 (PRDM16) is also called Transcription factor MEL1 (MDS1/EVI1-like gene 1). PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. PRDM16 is expressed in the uterus and kidney. PRDM16 regulates transcription through DNA binding and plays a role in the differentiation of myoblastic precursors into brown adipose tissue (BAT), and represses TGF-beta signalling. Translocations in PRDM16 occur in subsets of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) cases.

Specificity

This antibody can recognize all four isoforms of PRDM16.

Immunogen

KLH-conjugated linear peptide corresponding to human PRDM16.

Application

Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PRDM16 in rat brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected PRDM16 in human brain cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 15 µg of rat brain tissue lysate.

Target description

~100 kDa, ~135 kDa and ~150 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms between ~120 kDa and ~140 kDa

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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