AB3402P

Anti-Agouti Related Protein Antibody

Chemicon^®, from rabbit

• Synonym(s): AGRP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; western blot: suitable
• NCBI accession no.: NM_001138.1; NM_007316.1
• UniProt accession no.: O00253
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... AGRP(181)

Description

General description

Agouti-related peptide-expressing (AgRP) neurons seem to be critical for the acute regulation of eating. The AgRP protein is a strong antagonis of MC3-R and MC4-R and is expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.

Specificity

Recognizes AGRP. The immunogen shows no significant sequence homology with Agouti, tubby, tub, TULP1 or TULP2.

SPECIES REACTIVITIES: The immunogen sequence is 85% homologous with human and bovine. Reactivity with other species has not been confirmed.

Immunogen

A 15 amino acid peptide near the C-terminus of mouse AGRP (Shutter et al. 1997; Ollmann et al. 1997).

Application

Detect Agouti Related Protein using this Anti-Agouti Related Protein Antibody validated for use in ELISA & WB.

Research Category
Metabolism

Research Sub Category
Glucose/Glycogen Metabolism

Western blot: 1-10 μg/mL using ECL.



ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Physical form

Liquid in phosphate buffered saline containing 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable