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Key Documents

AB10545

Sigma-Aldrich

Anti-TIGAR Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

chromosome 12 open reading frame 5, TP53-induced glycolysis and apoptosis regulator, transactivated by NS3TP2 protein, probable fructose-2,6-bisphosphatase TIGAR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

bovine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_065108

UniProt accession no.

Q9NQ88

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TIGAR(57103)

General description

TIGAR (TP53-induced glycolysis and apoptosis regulator) is a p53-inducible protein that is thought to diminish levels of reactive oxygen species (ROS) and this may also contribute to p53 protecting from genomic damage. TIGAR’s ability to lower ROS levels may also have an important role to play in the autophagic response. It is known to decrease levels of fructose-2,6-bisphosphate and in turn inhibits glycolysis. Under ischemic stress, recent research has shown that TIGAR and p53 can regulate cellular energy homeostasis and apoptosis.

Immunogen

KLH-conjugated linear peptide corresponding to human TIGAR.

Application

Anti-TIGAR Antibody is an antibody against TIGAR for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected TIGAR in normal human brain and glioblastoma cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected TIGAR in 10 µg of HeLa cell lysate.

Target description

~30 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eric C Cheung et al.
Cancer cell, 37(2), 168-182 (2020-01-28)
The TIGAR protein has antioxidant activity that supports intestinal tissue repair and adenoma development. Using a pancreatic ductal adenocarcinoma (PDAC) model, we show that reactive oxygen species (ROS) regulation by TIGAR supports premalignant tumor initiation while restricting metastasis. Increased ROS
Mark D Wilkie et al.
Cancer letters, 478, 107-121 (2020-03-03)
Patients with mutated TP53 have been identified as having comparatively poor outcomes compared to those retaining wild-type p53 in many cancers, including squamous cell carcinomas of the head and neck (SCCHN). We have examined the role of p53 in regulation
Yan Tang et al.
eLife, 11 (2022-03-08)
Cholinergic and sympathetic counter-regulatory networks control numerous physiological functions, including learning/memory/cognition, stress responsiveness, blood pressure, heart rate, and energy balance. As neurons primarily utilize glucose as their primary metabolic energy source, we generated mice with increased glycolysis in cholinergic neurons

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