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251760

Sigma-Aldrich

DAR-4M

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

Synonym(s):

DAR-4M, Diaminorhodamine-4M

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About This Item

Empirical Formula (Hill Notation):
C25H26N4O3
Molecular Weight:
430.50
UNSPSC Code:
12352202
NACRES:
NA.25

Quality Level

Assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

red-violet

fluorescence

λex ~560 nm
λem ~575 nm

shipped in

ambient

storage temp.

−20°C

General description

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Irritant (B)

Physical form

In DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Legal Information

Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C


Certificates of Analysis (COA)

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