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Sigma-Aldrich

Anti-Collagen Type X Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... COL10A1(1300)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes type X collagen.
Recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
This Anti-Collagen Type X Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Collagen Type X.

Immunogen

Rat
purified, rat chondrosarcoma cell collagen type X

Application

Frozen Sections (see comments)

Immunoblotting (1:100-1:300)

Immunoprecipitation (1:20-1:40)

Paraffin Sections (see application reference; pretreatment required)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Chung, K.S., et al. 1995. Dev. Biol. 170, 387.
Schmid. T.M., and Linsenmayer, T.M. 1985. Dev. Biol. 107, 373.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Karthikeyan Rajagopal et al.
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Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 29(8), 1815-1822 (2014-03-20)
Activating mutations in transient receptor potential vanilloid family member 4 (Trpv4) are known to cause a spectrum of skeletal dysplasias ranging from autosomal dominant brachyolmia to lethal metatropic dysplasia. To develop an animal model of these disorders, we created transgenic
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Extracellular matrix (ECM) and its architecture have a vital role in articular cartilage (AC) structure and function. We hypothesized that a multi-layered chitosan-gelatin (CG) scaffold that resembles ECM, as well as native collagen architecture of AC, will achieve superior chondrogenesis
Marjolein M J Caron et al.
PloS one, 11(4), e0153162-e0153162 (2016-04-07)
NSAIDs are used to relieve pain and decrease inflammation by inhibition of cyclooxygenase (COX)-catalyzed prostaglandin (PG) synthesis. PGs are fatty acid mediators involved in cartilage homeostasis, however the action of their synthesizing COX-enzymes in cartilage differentiation is not well understood.

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