208731

Calpain Substrate II, Fluorogenic

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

• Synonym(s): Calpain Substrate II, Fluorogenic, Suc-LY-AMC
• Empirical Formula (Hill Notation): C₂₉H₃₁N₃O₇
• CAS Number: 94367-20-1
• Molecular Weight: 533.57
• MDL number: MFCD00057900
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: off-white
• solubility: DMSO: soluble
• fluorescence: λ_ex ~380 nm; λ_em ~460 nm
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
• InChI key: VSWNAJAOJZXWBM-FTJGSZSVSA-N

Description

General description

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Suc-Leu-Tyr-AMC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable