189738

Streptavidin, TEXAS RED^® Conjugate

This Streptavidin, TEXAS RED^® Conjugate is validated for use in ELISA, Flow Cytometry, Immunohistochemistry.

• UNSPSC Code: 41116133
• NACRES: NA.47

Properties

• Quality Level: 100
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze; protect from light
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Streptavidin conjugated to TEXAS RED^® fluorescent dye. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Application

ELISA (1:2000-1:5000)

Flow Cytometry (1:50-1:200)

Immunohistochemistry (1:50-1:200)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.

Reconstitution

Do not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.

Other Notes

Biotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 10⁶ cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Texas Red is a registered trademark of Life Technologies

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable