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2,3,5-Triphenyltetrazolium chloride

Color indicator. Supplement in culture media for the microbiological analysis of food and water samples

Synonym(s):

2,3,5-Triphenyltetrazolium chloride, TTC, TT, TPTZ, TTZ, Tetrazolium Red

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About This Item

Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
298-96-4
Molecular Weight:
334.80
Beilstein:
3923356
MDL number:
MFCD00011963
UNSPSC Code:
41106212
NACRES:
NA.74

Quality Level

200

Assay

≥98% (calculated on dried substance, perchloric acid titration)

form

solid

pH

3.7 (20 °C, 10 g/L in H2O)

mp

243 °C (decomposition)

solubility

150 g/L

bulk density

230 kg/m3

application(s)

microbiology

storage temp.

2-30°C

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

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General description

2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. Live microorganisms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells.

Application

2,3,5-triphenyltetrazolium chloride (TTC) is an indicator recommended for the microbiological analysis of food.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): ≥ 98 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λmax. (water): 245 - 248 nm
Spec. Absorptivity A 1%/1cm (λmax; 0,01 g/l; water; calculated on dried substance): ≥ 770
TLC-Test: passes test
Loss on drying (70 °C; ≤ 1 hPa): ≤ 0.5 %
Suitability for testing the germinability of seeds: passes test
Suitability for microbiology: passes test

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H228 - H315 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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