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V7754

Sigma-Aldrich

V5 Peptide

≥97% (HPLC), lyophilized powder

Synonym(s):

V5 Tag Peptide

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About This Item

Empirical Formula (Hill Notation):
C67H113N17O21S
CAS Number:
Molecular Weight:
1524.78
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.56

product name

V5 Peptide, ≥97% (HPLC), lyophilized powder

Quality Level

Assay

≥97% (HPLC)

form

lyophilized powder

mol wt

calculated mol wt 1524.78 Da

storage temp.

−20°C

Amino Acid Sequence

Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

General description

V5 Tag Peptide is a synthetic peptide that has an amino acid sequence correspondinh to amino acids 95-108 of the non-structural protein V and to the RNA polymerase a subunit (P protein), of Paramyxovirus SV5 with an N-terminal cysteine.

Application

The peptide is useful for displacement of V5-tagged fusion proteins from anti-V5 antibody in immunoassays. The successful inhibition of antibody binding of V5 peptide demonstrates binding is specific.

Other Notes

The amino acid sequence corresponds to amino acids 95-109 of non-structural protein V and to RNA polymerase α subunit (P protein), of Paramyxovirus SV5 with an N-terminal cysteine.
Amino acid sequence is N-Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-C
Lyophilized from 0.1% TFA in H2O

Preparation Note

To prepare a stock solution, dissolve in water to a final concentration of 5 mg/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eden A Dejene et al.
The Journal of biological chemistry, 295(30), 10255-10270 (2020-05-28)
Poly(A)-specific ribonuclease (PARN) is a 3'-exoribonuclease that plays an important role in regulating the stability and maturation of RNAs. Recently, PARN has been found to regulate the maturation of the human telomerase RNA component (hTR), a noncoding RNA required for
Yan-ling Zhang et al.
Archives of biochemistry and biophysics, 483(1), 45-54 (2009-01-06)
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Cell chemical biology, 27(1), 105-121 (2019-12-31)
RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. Here we investigated the molecular and phenotypic role of RUVBL1/2 ATPase activity in non-small cell lung cancer (NSCLC). We find that RUVBL1/2
Rui Chen et al.
PloS one, 12(12), e0190241-e0190241 (2017-12-28)
Survival of cancer cells in the harsh tumor microenvironment, characterized by oxygen and glucose deprivation, requires rapid initiation of cytoprotective measures. Metabolites whose levels change during stress are ideal signaling cues, particularly if used in post-translational modifications of stress-responsive signal

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