U1500
Urease from Canavalia ensiformis (Jack bean)
Type III, powder, 15,000-50,000 units/g solid
Synonym(s):
Jack bean urease, Urea amidohydrolase
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About This Item
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biological source
Canavalia ensiformis
type
Type III
form
powder
specific activity
15,000-50,000 units/g solid
mol wt
~544620 Da
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
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General description
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.
Biochem/physiol Actions
Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.
Unit Definition
One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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