Skip to Content
Merck
All Photos(1)

Documents

T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

Synonym(s):

TMB membrane substrate

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, ready to use solution

Quality Level

form

liquid

storage temp.

2-8°C

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used as a chromogenic substrate in western blot analysis. It has also been used as a liquid substrate in enzyme-linked immunosorbent assay (ELISA).
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
  • enzyme-linked immune absorbent spot (ELISPOT) assay
  • as a substrate for IgG peroxidase
  • in the visualization of immunocomplexes

Physical form

Ready-to-use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Muhammed M Salahuddin et al.
Current issues in molecular biology, 43(3), 2199-2209 (2021-12-24)
Breast cancer is the most common malignancy in women worldwide. P2X7 is a transmembrane receptor expressed in breast cancer and activated by the ATP tumor microenvironment, driving cell proliferation, angiogenesis, and metastasis via different signaling pathways. The role of the
Srdjan M Dragovic et al.
Cell host & microbe, 23(4), 523-535 (2018-04-13)
Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against
Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus
Berdun S, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(10), G801-G806 (2015)
Hesham Saeed et al.
Protein expression and purification, 181, 105820-105820 (2021-01-14)
In previous studies Pseudomonas aeruginosal-ASNase complete coding sequence gene, 984 bp (GenBank accession number KU161101.2) was isolated by PCR, cloned into pET28a(+) vector, expressed in E. coli DE3(BL21) pLysS, purified to apparent homogeneity and biochemically characterized. In the present work
Navilla Apú et al.
Open life sciences, 18(1), 20220577-20220577 (2023-08-17)
Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use enzyme-linked immunosorbent assays (ELISA) or chemiluminescence immunoassays (CLIA); however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service