RAB0105

Human Cystatin C ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): Cystatin-3, Cystatin-C
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... CST3(1471)
• Input: sample type plasma; sample type urine; sample type serum; sample type cell culture supernatant(s)
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: dry ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type plasma; sample type urine; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 300 pg/mL; standard curve range: 0.313-20 ng/mL
• detection method: colorimetric
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CST3(1471)

Description

General description

The Human Cystatin C ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human cystatin C in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CystatinC

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Cystatin C (CysC) is released by all nucleated cells. Abnormal thyroid function, immunosuppressive therapy and systemic inflammation alter the levels of CysC. Mutation in the gene encoding CysC causes hereditary cystatin C amyloid angiopathy, which is a consequence of amyloid deposits containing the CysC variant in the brain.

Cystatin-C precursor (CST3) is an inhibitor of lysosomal cysteine proteases[1] and several cathepsins like cathepsin S, a lysosomal enzyme.[2] It has been shown that CST3 is a marker of glomerular filtration and diabetic kidney disease.[3] In the presence of pathological conditions, it forms amyloid fibrils in brain arteries.[4] Mutations in the gene encoding CST3 have been shown to be associated with a form of amyloidosis[1] and age-related macular degeneration.[2]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids