The GlycoProfile™ β-Elimination kit contains a novel non-reducing reagent to efficiently and specifically remove O-linked carbohydrates from glycoproteins with minimal protein or glycan destruction. Consequently, additional downstream proteomics and glycomic analyses may be employed.
Quantity
The kit includes the reagents and techware for O-deglycosylation of up to 24 samples (200 uL at 1-10 ug/uL of glycoprotein per sample).
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
Sodium hydroxide solution, 5.0 M 60 μL
Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea
Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)
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