P3375

Papain from papaya latex

crude powder, 1.5-10 units/mg solid

• Synonym(s): Papainase
• CAS Number: 9001-73-4
• UNSPSC Code: 12352204
• eCl@ss: 42030311
• EC Number: 232-627-2
• NACRES: NA.54
• MDL number: MFCD00131791
• EC Number: 3.4.22.2 (BRENDA, IUBMB)
• Specific activity: 1.5-10 units/mg solid

Properties

• form: crude powder
• Quality Segment: 200
• specific activity: 1.5-10 units/mg solid
• mol wt: 23.4 kDa
• storage temp.: 2-8°C

Description

Application

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Papain from papaya latex has been used:

• in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
• in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B₁ molecules
• as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.

Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Analysis Note

Not standardized with lactose or other adulterants.

Other Notes

One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves