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P2783

Sigma-Aldrich

Phosphotransacetylase from Bacillus stearothermophilus

lyophilized powder, ≥3,000 units/mg protein (biuret)

Synonym(s):

Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase

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About This Item

CAS Number:
9029-91-8
Enzyme Commission number:
2.3.1.8 (BRENDA, IUBMB)
EC Number:
232-855-2
MDL number:
MFCD00131907
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥3,000 units/mg protein (biuret)

composition

Protein, ≥20%

shipped in

wet ice

storage temp.

−20°C

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Application

Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate. It is used to study metabolic pathways in various bacterium.

Biochem/physiol Actions

Phosphotransacetylase converts CoA to acetyl CoA.

Unit Definition

One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.

Physical form

Lyophilized powder containing potassium phosphate

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate
Philip R. Mallinder and Anne Moir
Microbiology, 137, 779-785 (1991)
G D Sprott et al.
Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)
Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in
Ikuo Sato et al.
Bioscience, biotechnology, and biochemistry, 74(1), 75-81 (2010-01-09)
Malic enzyme (ME) was purified as an electrophoretically homogenous protein from Rhodopseudomonas palustris No. 7. The molecular weight of ME was estimated to be 650 kDa and that of its subunit, 86 kDa. ME activity was remarkably enhanced by di-
Ling Jiang et al.
Biotechnology and bioengineering, 108(1), 31-40 (2010-09-09)
Repeated fed-batch fermentation of glucose by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor (FBB) was successfully employed to produce butyric acid at a high final concentration as well as to adapt a butyric-acid-tolerant strain. At the end of the
Maria Dimou et al.
Molecular biology reports, 38(6), 3653-3663 (2010-11-26)
Acetate is abundant in soil contributing to a great extent on carbon cycling in nature. Phosphate acetyltransferase (Pta, EC 2.3.1.8) catalyzes the reversible transfer of the acetyl group from acetyl-P to CoA forming acetyl-CoA and inorganic phosphate, participating to acetate
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