N7653
p-Nitrophenyl Phosphate Liquid Substrate System
alkaline phosphatase substrate, liquid
Synonym(s):
4-Nitrophenyl phosphate disodium salt solution, pNPP
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All Photos(3)
About This Item
Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
Molecular Weight:
263.05
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
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Product Name
p-Nitrophenyl Phosphate Liquid Substrate System, liquid
form
liquid
Quality Level
shipped in
wet ice
storage temp.
−20°C
SMILES string
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
General description
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
Application
p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.
Caution
Stable at least one year when stored below 0 °C.
Physical form
Ready-to-use.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
Target Organs
Kidney,Liver,Blood
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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