MTOX1304

Canine MDR1 Knockout, Human BCRP Knockin MDCKII Cells

canine kidney (cocker spaniel)

• Synonym(s): MDCKII MDR1 KO hBCRP knockin
• UNSPSC Code: 12352207
• NACRES: NA.81
• Biological source: canine kidney (cocker spaniel)

Properties

• Product Name: Canine MDR1 Knockout, Human BCRP Knockin MDCKII Cells,
• biological source: canine kidney (cocker spaniel)
• Quality Segment: 200
• form: liquid
• storage temp.: −196°C
• Gene Information: human ... ABCG2(9429)

Description

Application

See technical bulletin for detailed protocols

Features and Benefits

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.

The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human BCRP has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard BCRP substrates.

Analysis Note

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

Exhibit 1: ADME/Tox cell lines license

MDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable