If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
MAK320
D-2-Hydroxyglutarate (D2HG) Assay Kit
sufficient for 200 fluorometric reactions
Synonym(s):
D2HG Test Kit
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| Size/SKU | Availability | Price |
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About This Item
usage
sufficient for 200 reactions (Fluorometric)
detection method
fluorometric
relevant disease(s)
cancer; neurological disorders
shipped in
wet ice
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Features and Benefits
- Highly sensitive and rapid enzymatic assay for the detection of D2HG levels in various biological fluids
- Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
- Quick instruction bench card - to assure your experimental success
- More experiments in one kit - contains sufficient reagents for 200 tests
- Detection time: only 30-60 minutes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| detection method fluorometric | detection method colorimetric | detection method - | detection method colorimetric, fluorometric |
| usage sufficient for 200 reactions (Fluorometric) | usage sufficient for 100 colorimetric tests | usage - | usage sufficient for 100 colorimetric or fluorometric tests |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| relevant disease(s) cancer; neurological disorders | relevant disease(s) cancer | relevant disease(s) - | relevant disease(s) cancer |
Storage Class
11 - Combustible Solids
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the recommended approach for measuring 2-HG levels in cell culture samples using the MAK320 detection kit if the sample volume is very small (i.e. 100 ul) and there is no access to equipment for accurately measuring pH in small volumes? Additionally, is the pH range critical for the experiment to work? Would a higher or lower pH result in decreased enzyme activity or render the experiment ineffective?
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The recommended solution is to use litmus paper to measure the pH of the sample. Another option is to use a special electrode designed for very small volumes, although this can be complicated and expensive. While the pH of the sample is crucial for test performance, the pH range is wide, making pH paper suitable and requiring only a few microliters of the sample.
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