When reconstituted and filter sterilized, this medium is expected to have a similar shelf-life as prepared 1X Medium 199 with Hanks salts and L-glutamine. See product 51322C for the prepared liquid medium. This product has an expiration date of 1 year from the date of quality release. Upon the addition of serum or other heat-sensitive components, the shelf life would be limited to 1 - 2 weeks at 2 - 8°C. Freeze/thaw cycles may result in precipitate formation.
M0393
Medium 199
With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
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Product Name
Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
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General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to test and determine the pH sensitivity of the sensors in a complex environment
- as a culture medium during oocyte harvesting
- as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
- to culture visceral adipose tissue explants
Quantity
Formulated to contain 10.6 grams of powder per liter of medium.
Reconstitution
Supplement with 0.35 g/L sodium bicarbonate.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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