GE17-5481-01

Capto^™ Core 700

Cytiva 17-5481-01, pack of 25 mL

• Synonym(s): Capto Core, Capto Core 700
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: octylamine
• packaging: pack of 25 mL
• manufacturer/tradename: Cytiva 17-5481-01
• matrix: highly cross-linked agarose
• avg. part. size: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning in place: 3-13(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
• working range: 2-14(pH interval where the medium can be operated without significant change in function.)
• capacity: ~13 mg binding capacity (ovalbumin)
• suitability: suitable for bioprocess medium

Description

General description

Dynamic binding capacities were measured at 10% breakthrough with a residence time of 3 min (1.6 ml/min = 200 cm/h) in HiScreen columns. Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality. This chromatography resin has both size exclusion and binding properties.

Multimodal removal of contaminants

Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.

Hydrophobic, positively charged ligands

The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.

Scaling up is simple

HiTrap and HiScreen columns can be connected in series for increased capacity and are convenient to use when developing an efficient and robust separation method.

Application

• Multimodal contaminate removal
• Virus and exosome purification
• Process development
• Biomolecule purification

Features and Benefits

• Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
• Significantly improved productivity and higher flow rates compared with standard size exclusion (gel filtration) methods.
• Straightforward optimization due to flowthrough chromatography and robust performance.
• Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
• Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids