GE17-1179-01
Resource™ Q
Cytiva 17-1179-01, pack of 6 mL
Synonym(s):
Resource Q Column
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About This Item
UNSPSC Code:
47101511
NACRES:
NA.56
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description
Ion Exchanger Type (value)
packaging
pack of 6 mL
manufacturer/tradename
Cytiva 17-1179-01
parameter
87 psi
bed size
16 mm × 30 mm
bed volume
6 mL
matrix
polystyrene/divinylbenzene
avg. part. size
15 μm
particle size
15-15 μm
cleaning
1-14
working range
2-12
suitability
suitable for bioprocess medium
Related Categories
General description
RESOURCE™ Q columns are prepacked with SOURCE™ 15Q, a strong anion exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules.
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Application
Automated pilot scale purification of synthetic oligonucleotides; AKTAexplorer Summary
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Features and Benefits
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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