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G2025

Sigma-Aldrich

Glycerol

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)

Synonym(s):

1,2,3-Propanetriol, Glycerin

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About This Item

Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
Molecular Weight:
92.09
Beilstein:
635685
EC Number:
MDL number:
UNSPSC Code:
50161901
PubChem Substance ID:
NACRES:
NA.21

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

product line

BioReagent

Assay

≥99% (GC)

form

viscous liquid

autoignition temp.

698 °F

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

≤0.005% Chlorinated compounds
≤0.005% Sulfate (SO4)

refractive index

n20/D 1.474 (lit.)

pH

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

density

1.25 g/mL (lit.)

suitability

suitable for electrophoresis

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol has been used as
  • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
  • a fuel during designing enzymatic biofuel cell.
  • a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • a matrix for fast atom bombardment MS.
  • may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.
Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

390.2 °F - Pensky-Martens closed cup

Flash Point(C)

199 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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