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Fluorescein di(β-D-galactopyranoside)

Name: Fluorescein di(β-<SC>D</SC>-galactopyranoside)
Brand: SIGMA

Synonym(s):

FDG

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About This Item

Empirical Formula (Hill Notation):

C₃₂H₃₂O₁₅

CAS Number:

17817-20-8

Molecular Weight:

656.59

NACRES:

NC.07

PubChem Substance ID:

24894829

UNSPSC Code:

12352201

MDL number:

MFCD00077344

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Properties

assay

≥98% (HPLC)

Quality Level

100

form

powder

solubility

methanol: 20 mg/mL, clear, yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1

InChI key

ZTOBILYWTYHOJB-WBCGDKOGSA-N

Description

General description

Fluorogenic substrate for β-galactosidase

Application

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).[1][2]

Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

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This Item	D0531	B9511	43188
Sigma-Aldrich F2756 Fluorescein di(β-D-galactopyranoside)	Sigma-Aldrich D0531 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride	Sigma-Aldrich B9511 5-Bromo-4-chloro-3-indolyl β-D-fucopyranoside	Sigma-Aldrich 43188 p-Nitrophenyl-β-D-ribofuranoside
form powder	form powder	form powder	form powder
solubility methanol: 20 mg/mL, clear, yellow	solubility -	solubility DMF: 20 mg/mL, clear, colorless to faintly yellow	solubility methanol: 50 mg/mL, clear, colorless to very faintly yellow
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 100
assay ≥98% (HPLC)	assay ≥90% (HPLC)	assay ≥98% (HPLC)	assay ≥98.0% (HPLC)
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Dual Somatostatin Receptor/FDG PET/CT Imaging in Metastatic Neuroendocrine Tumours: Proposal for a Novel Grading Scheme with Prognostic Significance.

David Lh Chan et al.

Theranostics, 7(5), 1149-1158 (2017-04-25)

A fluorimetric method using fluorescein di-?-D-galactopyranoside for quantifying the senescence-associated ?-galactosidase activity in human foreskin fibroblast Hs68 cells.

Yang NC and Hu ML

Analytical Biochemistry, 325, 337-343 (2004)

Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.

Plovins A

Applied and Environmental Microbiology, 60, 4638-4638 (1994)

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Global Trade Item Number

SKU	GTIN
F2756-1MG	04061833613009
F2756-5MG	04061833613290
F2756-25MG	04061833613269

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