D6815

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

peroxidase substrate, liquid, suitable for membrane applications

• Synonym(s): DAB membrane substrate
• UNSPSC Code: 12352204
• PubChem Substance ID: 24894054
• NACRES: NA.83
• MDL number: MFCD00012969

Properties

• Product Name: 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride, for Membrane Applications
• Quality Segment: 200
• form: liquid
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChI key: KJDSORYAHBAGPP-UHFFFAOYSA-N

Description

Application

The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.

• in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining
• in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi
• in immunoperoxidase staining of lung tissue

Biochem/physiol Actions

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.

Packaging

Set consists of 4 x 100 ml Solution A and 15 ml Solution B.

Preparation Note

Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

Disclaimer

Stable for up to 18 mo. when stored at 2-8 °C in the original container.

Kit Components Also Available Separately

• 3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100

  SDS

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H341,H350
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B - Muta. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects