CGP1
Glutathione Peroxidase Cellular Activity Assay Kit
Sufficient for 100 colorimetric tests
Synonym(s):
Cellular glutathione peroxidase, c-GPx
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About This Item
NACRES:
NA.32
UNSPSC Code:
12161503
Quality Level
usage
sufficient for 100 tests
shelf life
Kit is stable for 24 months when unopened.
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
Application
Suitable for the measureent of glutathione peroxidase in tissue extracts
Biochem/physiol Actions
The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
Other Notes
This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.
Kit Components Only
Product No.
Description
- tert-Butyl hydroperoxide 1 mL
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
5.2 - Organic peroxides and self-reacting hazardous materials
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| CGP1-1KT | 04061833531761 |